Zeiss PALM MicroBeam Laser Microdissection Microscope

BioCRF

The Hong Kong University of Science and Technology

 

Microscope:

Axio Observer 7 with stage insert for

  1. glass slides (maximum length: 78mm)

  2. 35mm and 50mm dishes

  3. 24-60mm dishes (universal)



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Magnification

Fluar 5x

Fluar 10x

LD Plan-Neofluar 20x

LD Plan-Neofluar 40x

LD Plan-Neofluar 63x

EC Plan-Neofluar 100x

NA

0.25

0.5

0.4Corr

0.6Corr

0.75Corr

1.3

Dry/Oil

Dry

Dry

Dry

Dry

Dry

Oil

Camera:

  1. 1.Axiocam 506 mono

  2. 2.Axiocam 506 color

Software:

PALM Robo 4.8 Pro (connected to WACOM PEN display DTK2451)

Cutting and Catapulting Laser:

CryLaS (355nm) UV laser

Objective Options:

Accessories:

Collectors:

  1. 8-tube collector (200μl and 500μl)

  2. Capture plate collector 96 (for use with capture plate 96)

  3. Cap collector 12×8

  4. Single tube collector 500RM and 200RM

  5. Single cap collector 500RM and 200RM

Position in Microscope

1


2



3


4

Filter Set

Laser


44



45


96

Dyes

n.a.


Alexa 488, eGFP, FITC, Fluorescein,

GFP, Oregon Green, SYBR Green I


Alexa 568, Cy 3.5, DsRed, MitoTracker Red


DAPI, BFP, Alexa 405

Filter Options:

Emission

n.a.


BP 530/50



BP 630/75


BP 450/40

Excitation

n.a.


BP 475/40



BP 560/40


BP 390/40

Consumables:

Slides:

  1. Membrane slide 1.0 PET

  2. Membrane slide 1.0 PEN


Collection Tubes:

  1. Muster microtube 500

  2. Microtube 500

  3. Adhesive cap 200 clear

  4. Adhesive cap 500 opaque


Live Cell Culturing and Cutting:

  1. Duplex dish (35mm and 50mm)

  2. Membrane ring (35mm and 50mm)


For price, please enquire.

Sample Requirement:

Applicable to cryosections and FFPE (formalin-fixed and paraffin embedded tissues)