MALDI TOF/TOF Mass Spectrometer

BioCRF

The Hong Kong University of Science and Technology

 

Description: MALDI TOF/TOF ultrafleXtremeTM

Mass Spectrometer


Manufactures: Bruker Daltonics


Link to manufacturer’s page: UltrafleXtreme


Services provided:

1.  Protein Identification

This service incorporates both peptide mass fingerprinting (PMF) and MS/MS peptide fragmentation. The sample (single protein spot/band) is digested with trypsin and the digested fragments are recorded. A list of fragments (“fingerprint”) is generated. From the list, the most abundant peptide ions are selected for MS/MS analysis. The fragmentation produces series of sequence ions that are used for database searching. The protein is identified by matching the experimental results with the theoretical fragmentation patterns in database using MASCOT.


Raw data can be viewed by software called “FlexAnalysis”.  PC workstation with the software installed is available for booking at our facility. HKUST intranet users can book the workstation through the online booking system.


    a) Full service for Protein Identification

          (with sample preparation)

          In-gel Digestion, Extraction,

          ZipTip® Desalting,

          MALDI-TOF/TOF (PMF+MS/MS) and

          Database search


     b) Protein Identification*

         (without sample preparation)

         MALDI-TOF/TOF (PMF+MS/MS) and

         Database search

          *Sample spotted on target plate


2.  Accurate Mass Measurement

This service provides the accurate mass measurement(s) of protein(s) in the sample.


     a) Accurate Mass Measurement

          ZipTip® Desalting and MALDI-TOF

          Manual export of MS spectrum and

          peak list data (in Excel format)


Sample Submission Guideline:

The facility is to be operated as a first-come-first-serve basis. 

Click here for more information


Contact: Ms. Joyce Wong

(E-mail: joycepswong@ust.hk)


Location: Room 6127A


Booking:

Please contact Ms. Joyce Wong for booking of the instrument.


Conditions of use:

General user policy

Other conditions:

♦     Please fill in the MS MALDI Request Form   in FULL and submit sample(s) during office hour.

     Charges




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