MGI DNBSEQ-T7 & DNBSEQ-G400 Sequencers
About
Standalone Sequencing Service and Full NGS Service are provided with our state-of-the art DNBSEQ sequencers.
Date of upcoming sequencing run: In the week of 25 Nov, 2024
Deadline for Standalone Sequencing Library submission: 18 Nov, 2024
Deadline for DNA/ RNA Full Service Sample submission: 13 Nov, 2024
Standalone Sequencing Service
Ready-to-sequence libraries of various applications such as RNA sequencing (RNA-seq), Whole Genome sequencing (WGS), Whole Exome Sequencing (WES), ChIP-Sequencing (ChIP-Seq), ATAC-sequencing (ATAC-seq), Whole Genome Bisulfite Sequencing (WGBS) and metagenomic sequencing are submitted and sequencing is performed.
The workflow is listed as follows:
Sample Submission
REMINDER: If you have plan to submit standalone sequencing libraries to BioCRF, you are advised to consult us on the index usage before library preparation to avoid duplicated usage of the same index in the same sequencing run.
# If libraires with Illumina adapters are submitted, an additional conversion step has to be performed by BioCRF.
* The total amount listed here is estimated from a library that needs 165 Gb data or less, more input will be needed proportionally when higher sequencing data output is desired.
^ Qubit assay is the recommended method of sample quantification. Consumables are available for check-out from BioCRF
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Full NGS Service
With our liquid handlers, MGISP-960, MGISP-Smart8 and MGISP-100, BioCRF provides Full NGS Service for RNA-seq and WGS from extraction/ library preparation to sequencing with an automated workflow at a rapid turnaround time with a quality guarantee of Q30 ≥80% . Bioinformatic analysis is then performed on our High-performance Computer Clusters. The workflows are listed as follows:
WGS/ RNA-seq from purified DNA/RNA:
Sample Submission
# Users can check out Qubit consumables from BioCRF. If Nanodrop has to be used, the required amount and concentration should be double of those listed above.
^ For lower sample input, please consult BioCRF
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Blood WGS:
Sample Submission
*Please avoid blood collection tubes with heparin
# Users are required to fill out a biorisk assessment form to ensure that the processing of the incoming blood samples is compliant with the university safety regulations
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How do I get equipment access and/or apply for specialized services (mass spectrometry, sequencing & tissue processing)?"First, you have to register for an account on our online booking system to become an authorized user. To get access to self-service equipment, all users have to be trained (and in some cases, assessed) so that they can follow standard operation procedures and be aware of necessary safety precautions. They can register for training sessions on our booking system. For equipment that requires operation assessment, instructors will inform users of the assessment criteria during the training. After gaining access, users can enter the corresponding rooms where the equipment is located in and the main office of BioCRF (Room 6127) 24/7, unless emergencies arise. As for applying for specialized services, please read the guidelines on the following pages: 1. Sequencing 2. Mass Spectrometry 3. Tissue Processing
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What rules must I be aware of as a BioCRF user?Only the specific authorized user (but not other untrained users from the same group or department) is allowed to use the facilities in BioCRF. When making equipment reservations, authorized users should abide by the booking restrictions and rules of the equipment. Authorized users should sign the log books as appropriate and report any issues to BioCRF staff. Authorized users should ensure that the equipment is clean and in good working order after use. Authorized users should download their experimental data from the server of BioCRF. USB sticks and hard disks on BioCRF computers (except computers designated for data analysis) are strictly prohibited. If users do not show up for their reservations, or if they arrive 15 minutes later than their reservation start time, they will receive a no-show warning. Users also receive a warning from misusing or damaging any equipment. Upon receiving two, their access to the equipment will be suspended for at least a month. Any cost of repair due to the misuse of equipment will be charged to the account of the user's supervisor. The supervisor should inform users about the University's safety and environmental policies, as well as safety statements, standards and procedures related to the user's experiment. The supervisor should also inform the user of any potential hazard and safety precaution. In case of unexpected machine malfunction, users might not be able to continue their experiments and have to reschedule. Affected reservations will be cancelled.
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How can I retrieve my experimental data on BioCRF computers and how does BioCRF manage these data?Users can refer to our Data Download Guide for instructions. BioCRF does not use or back up users’ experimental data and we delete them periodically, users should download their files within 7 days.
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How to acknowledge BioCRF in my work?With the proper acknowledgement of the use of our instruments and services in your publications (reports, presentations, papers, etc.) and grant applications, we get to measure our impact on the research community and assess if our resources are efficiently utilized. This metric is important for the continued funding and support of our operations and expansion - so that you could enjoy more high-quality instruments and services! Any use of our resources should be acknowledged. Please acknowledge us as “Biosciences Central Research Facility, HKUST (Clear Water Bay)” in grant applications and the acknowledgement section of any publications. Example: We thank Biosciences Central Research Facility of HKUST (Clear Water Bay) to support the work in [flow cytometry/ histology/ general equipment/ genomics/ proteomics/ microscopy/ tissue culture*] core(s). *Please select the appropriate options Online form link: https://ust.az1.qualtrics.com/jfe/form/SV_6rINfENc4b1YWeq