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Proteomics & Metabolomics
The core provides a range of equipment - from protein imaging systems to mass-spectrometers - to support the study of proteomics and metabolomics.
Staff in charge: Ms. Joyce Wong and Ms. Haity Ngan
Liquid Chromatography & Mass Spectrometry
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ÄKTAavant is a preparative liquid chromatography system intended for protein sample purification. On the front side of the instrument, ÄKTAavant has a built-in fraction collector with a cooling functionality that prevents overheating of purified samples.
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The Orbitrap Eclipse incorporates the latest inventions in ion transmission and control, extended m/z range, and real-time decision-making. These new functionalities provide high sensitivity, selectivity, and versatility for obtaining comprehensive results from proteomic experiments.
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The timsTOF Pro achieves near 100% duty cycle for shotgun proteomics, by using the Parallel Accumulation Serial Fragmentation (PASEF) acquisition method in its dual TIMS (Trapped Ion Mobility Spectrometry) analyzer.
Metabolomics Analyzers
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Seahorse XFp Analyzers measure Oxygen Consumption Rate (OCR) and Extracellular Acidification Rate (ECAR) of live cells in an 8-well miniplate format. Its quick and easy setup makes it ideal for performing routine measurements of ex-vivo metabolic phenotype and other quantity-limited samples.
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The Biolog OmniLog Phenotype MicroArray (PM) Incubator Reader allows users to do PM for microbial and mammalian cells.
Protein Imaging & Western Systems
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Milo™ can measure protein expression of around 1000 single cells in a single run, in which the heterogeneity of the samples can be profiled.
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Odyssey is an infrared laser-based instrument for conducting Western blotting without the need for films, a darkroom, or any chemiluminescent substrates. It also offers low background fluorescence, enabling the detection of low-picogram targets with near-infrared fluorescence.
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Sally Sue™ let users separate and analyze proteins by size from 2-440kDa. Users can load as little as 5μl of samples with a protein concentration of 0.2μg/μl, and run up to 96 samples in one experiment.
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The ChemiDoc Touch system is a compact instrument for quantitative imaging of gels and membranes. It can detect a wide range of dyes and fluorophores.